名稱(chēng) | CCR5/Ga15/CHO |
型號(hào) | CBP71057 |
報(bào)價(jià) | ![]() |
特點(diǎn) | CCR5/Ga15/CHO 凍存條件:90% FBS+10% DMSO |
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藥靶細(xì)胞株 > GPCR細(xì)胞株 > CBP71057CCR5/Ga15/CHO

- 詳細(xì)內(nèi)容
CBP71057 | |
I. Background | |
CCR5 (chemokine (C-C motif) receptor 5) is a receptor for C-C type chemokines MIP-1α, MIP-1? and CCL5. CCR5 plays a role in the control of granulocytic lineage proliferation or differentiation. It also acts as co-receptor with CD4 for primary non-syncytium-inducing strains (macrophage-tropic) of HIV-1 virus. | |
II. Introduction | |
Host Cell: | CHO |
Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Gene Synonyms: | C-C chemokine receptor type 5, CCR5 |
Expressed Gene: | GenBank accession number NM_000579; no expressed tags |
Host Cell: | CHO-K1 |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | F-12+10%FBS+3μg/ml puromycin+100μg/ml Hygromycin B |
Mycoplasma Status: | Negative |
Storage: | Liquid nitrogen immediately upon delivery |
Application(s): | Functional assay for CCR5 receptor |
Transducer: | Gi |
Ⅲ. Description of Host Cell Line | |
Organism: | Hamster |
Tissue: | Ovary |
Morphology: | Epithelial |
Growth Properties: | Adherent |
Ⅳ. Representative Data | |
Concentration-dependent stimulation of intracellular calcium mobilization by MIP-1α in CCR5/Gα15/CHO and Gα15/CHO cells Figure 1. MIP-1α-induced concentration-dependent stimulation of intracellular calcium mobilization in CCR5/Gα15/CHO and Gα15/CHO cells. The cells were loaded with Calcium-4 prior to stimulation with an CCR5 receptor agonist, MIP-1α. The intracellular calcium change was measured by FlexStation. |